Journal: International Journal of Nanomedicine

Article Title: Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds

doi: 10.2147/IJN.S202402

Figure Lengend Snippet: SEM image and fiber size distribution of lidocaine/hEGF-loaded nanofibers. Abbreviations: SEM, scanning electron microscope; hEGF, human epidermal growth factor.

Article Snippet: An hEGF ELISA kit (Cusabio Biotech., USA) measured hEGF in the eluent via absorbance at 450 nm.

Techniques: Microscopy

Journal: International Journal of Nanomedicine

Article Title: Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds

doi: 10.2147/IJN.S202402

Figure Lengend Snippet: FTIR spectra of electrospun pure PLGA and lidocaine/hEGF-loaded PLGA nanofibers. Abbreviations: FTIR- fourier-transform infrared spectroscopy; hEGF, human epidermal growth factor; PLGA, poly[(d,l)-lactide-co-glycolide]; FTIR, fourier-transform infrared spectroscopy.

Article Snippet: An hEGF ELISA kit (Cusabio Biotech., USA) measured hEGF in the eluent via absorbance at 450 nm.

Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy

Journal: International Journal of Nanomedicine

Article Title: Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds

doi: 10.2147/IJN.S202402

Figure Lengend Snippet: Measured contact angles. ( A ) Pure PLGA nanofibers, 127.8, ( B ) lidocaine-incorporated nanofibers, 52.3, and ( C ) lidocaine/hEGF-loaded nanofibers, 60.1. Abbreviations: hEGF, human epidermal growth factor; PLGA, poly[(d,l)-lactide-co-glycolide].

Article Snippet: An hEGF ELISA kit (Cusabio Biotech., USA) measured hEGF in the eluent via absorbance at 450 nm.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds

doi: 10.2147/IJN.S202402

Figure Lengend Snippet: Stress–strain curve of pure PLGA, lidocaine-incorporated, and lidocaine/hEGF-loaded sheath-core-structured nanofibers. Abbreviations : hEGF, human epidermal growth factor; PLGA, poly[(d,l)-lactide-co-glycolide].

Article Snippet: An hEGF ELISA kit (Cusabio Biotech., USA) measured hEGF in the eluent via absorbance at 450 nm.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds

doi: 10.2147/IJN.S202402

Figure Lengend Snippet: In vitro elution profiles of hEGF from the nanofibrous films. Abbreviation: hEGF, human epidermal growth factor.

Article Snippet: An hEGF ELISA kit (Cusabio Biotech., USA) measured hEGF in the eluent via absorbance at 450 nm.

Techniques: In Vitro

Journal: International Journal of Nanomedicine

Article Title: Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds

doi: 10.2147/IJN.S202402

Figure Lengend Snippet: In vivo release of hEGF from the nanofibrous films. Abbreviation: hEGF, human epidermal growth factor.

Article Snippet: An hEGF ELISA kit (Cusabio Biotech., USA) measured hEGF in the eluent via absorbance at 450 nm.

Techniques: In Vivo

Journal: International Journal of Nanomedicine

Article Title: Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds

doi: 10.2147/IJN.S202402

Figure Lengend Snippet: Cell viability of electrospun lidocaine/hEGF nanofibers ( ## P <0.01, ** P <0.01). Abbreviations: hEGF, human epidermal growth factor; EGF, epidermal growth factor.

Article Snippet: An hEGF ELISA kit (Cusabio Biotech., USA) measured hEGF in the eluent via absorbance at 450 nm.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds

doi: 10.2147/IJN.S202402

Figure Lengend Snippet: Wound healing in hEGF/lidocaine-incorporated nanofibers at post-surgical days 1 and 3 (scale bar: 1 mm). A foreign body reaction was noted at day 1 ( D1 ), while fibroblastic proliferation and mixed infiltrates of lymphocytes and plasma cells were observed in the subcutis and muscular layer on day 3 ( D3 ). Abbreviation: hEGF, human epidermal growth factor.

Article Snippet: An hEGF ELISA kit (Cusabio Biotech., USA) measured hEGF in the eluent via absorbance at 450 nm.

Techniques: Clinical Proteomics

Journal: Fluids and Barriers of the CNS

Article Title: Blood-brain barrier permeability increases with the differentiation of glioblastoma cells in vitro

doi: 10.1186/s12987-024-00590-0

Figure Lengend Snippet: Identification of cytokines released by patient-derived glioblastoma cells. a . Cytokine levels in the medium of differentiated/adherent cells (AC) or stem cell/neurospheres (NS) of GBM cells derived from patients #1, #2 and #3, evaluated by a chemiluminescence-based array. The figure is representative of one out of three experiments with similar results. Squares identify the differentially expressed cytokines common to all patients. Red: IL-6; yellow: IL-8, TNF-α, MCP-1. b . ELISA detection of IL-6, IL-8, TNF-α, MCP-1 in AC- or NS-derived medium. Data were presented as mean ± SD ( n = 3 independent experiments; each experimental point was performed in technical duplicates). *** p < 0.001: AC vs. NS

Article Snippet: The levels of chemokine (C-C motif) ligand 2/monocyte chemoattractant protein 1 (CCL2/MCP-1), chemokine (C-X-C motif) ligand 8/interleukin-8 (CXCL8/IL-8), tumor necrosis factor-α (TNF-α) and IL-6 were measured by ELISA, using the Human MCP-1 (CCL2) Standard ABTS ELISA Kit (#900-K31, ImmunoTools, Friesoythe Germany), the Human IL-8 Elisa Kit (#31670089, ImmunoTools), the TNF-α ELISA kit (#31673019, ImmunoTools) and the Human IL-6 ELISA Kit High Sensitivity (#ab4604, Abcam).

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Plant Biotechnology

Article Title: Production of human epidermal growth factor in cell suspension cultures of Centella asiatica (L.) Urban using balloon-type bubble bioreactors

doi: 10.5010/jpb.2024.51.020.206

Figure Lengend Snippet: Fig. 1 Schematic representations of genetic constructs for allowing cytoplasmic hEGF accumulation or endoplasmic retention signal, hEGF-KDEL, under the control of the CaMV 35S promoter. A. Expression cassettes used for Centella asiatica transformation. B. PCR analysis of gDNA extracted from putative transgenic callus lines of C. asiatica. The PCR mixture was prepared by mixing 3 µl diluted gDNA and 2 µl primer set in Prime Taq Pre-mix (2X). Electrophoresis was performed using a 1.5% agarose gel for 60 min at 50 V. Lane M: DNA ladder; Lane E3 to E11, hEGF transgenic callus lines; Lane EK1-EK7, hEGF-KDEL transgenic callus lines; hEGF, human epidermal growth factor; PCR, polymerase chain reaction; gDNA, genomic DNA

Article Snippet: ELISA assay The protein samples were diluted at a ratio of 1:20 before each assay and were evaluated using a commercially available hEGF ELISA kit (R&D Systems Inc., USA) according to the manufacturer’s instructions.

Techniques: Construct, Control, Expressing, Transformation Assay, Transgenic Assay, Electrophoresis, Agarose Gel Electrophoresis, Polymerase Chain Reaction

Journal: Journal of Plant Biotechnology

Article Title: Production of human epidermal growth factor in cell suspension cultures of Centella asiatica (L.) Urban using balloon-type bubble bioreactors

doi: 10.5010/jpb.2024.51.020.206

Figure Lengend Snippet: Fig. 2 Transgenic hEGF and hEGF-KDEL cell lines of Centella asiatica. hEGF expression and growth was determined after 4 weeks of culture. Quantitative reverse transcription-polymerase chain reaction analysis was performed by adding 10 μL of TB green Premix Ex Taq, 1 μL of forward primer and reverse primer, 6 μL of dH2O, and 2 μL of cDNA to PCR tubes. zDifferent letters indicate significant differences at p < 0.05 according to Duncan’s multiple range test (n = 3 with hEGF and hEGF-KDEL lines). hEGF, human epidermal growth factor

Article Snippet: ELISA assay The protein samples were diluted at a ratio of 1:20 before each assay and were evaluated using a commercially available hEGF ELISA kit (R&D Systems Inc., USA) according to the manufacturer’s instructions.

Techniques: Transgenic Assay, Expressing, Reverse Transcription, Polymerase Chain Reaction

Journal: Journal of Plant Biotechnology

Article Title: Production of human epidermal growth factor in cell suspension cultures of Centella asiatica (L.) Urban using balloon-type bubble bioreactors

doi: 10.5010/jpb.2024.51.020.206

Figure Lengend Snippet: Fig. 3 Western blot analysis of transgenic hEGF and hEGF-KDEL cell lines of Centella asiatica. A. Immunoblotting of hEGF protein. B. hEGF concentration [M, marker; WT, non-transformed callus; PC, positive control (200 ng of recombinant hEGF protein obtained from Sigma-Aldrich)]. (n = 3 with each analysis). hEGF, human epidermal growth factor

Article Snippet: ELISA assay The protein samples were diluted at a ratio of 1:20 before each assay and were evaluated using a commercially available hEGF ELISA kit (R&D Systems Inc., USA) according to the manufacturer’s instructions.

Techniques: Western Blot, Transgenic Assay, Concentration Assay, Marker, Transformation Assay, Positive Control, Recombinant

Journal: Journal of Plant Biotechnology

Article Title: Production of human epidermal growth factor in cell suspension cultures of Centella asiatica (L.) Urban using balloon-type bubble bioreactors

doi: 10.5010/jpb.2024.51.020.206

Figure Lengend Snippet: Fig. 4 Cell cycle analysis of hEGF and hEGF-KDEL transgenic lines via flow cytometry. G0/G1, Gap1 phase; S, DNA synthesis phase; G2/M, Gap2 phase and metaphase (n = 3 with each analysis). hEGF, human epidermal growth factor

Article Snippet: ELISA assay The protein samples were diluted at a ratio of 1:20 before each assay and were evaluated using a commercially available hEGF ELISA kit (R&D Systems Inc., USA) according to the manufacturer’s instructions.

Techniques: Cell Cycle Assay, Transgenic Assay, Flow Cytometry, DNA Synthesis

Journal: Journal of Plant Biotechnology

Article Title: Production of human epidermal growth factor in cell suspension cultures of Centella asiatica (L.) Urban using balloon-type bubble bioreactors

doi: 10.5010/jpb.2024.51.020.206

Figure Lengend Snippet: Fig. 5 Correlation analysis of hEGF expression with fresh weight of the cells, total protein content, and ratio of cells which were in G2/M phases of the cell cycle. Gene expression level was determined via quantitative reverse transcription-polymerase chain reaction. hEGF, human epidermal growth factor

Article Snippet: ELISA assay The protein samples were diluted at a ratio of 1:20 before each assay and were evaluated using a commercially available hEGF ELISA kit (R&D Systems Inc., USA) according to the manufacturer’s instructions.

Techniques: Expressing, Gene Expression, Reverse Transcription, Polymerase Chain Reaction

Journal: Journal of Plant Biotechnology

Article Title: Production of human epidermal growth factor in cell suspension cultures of Centella asiatica (L.) Urban using balloon-type bubble bioreactors

doi: 10.5010/jpb.2024.51.020.206

Figure Lengend Snippet: Fig. 6 Effect of incubation in the dark and with exposure to different light conditions on the proportion of cells which were in G2/M phases during cell suspension cultures of transgenic EK2 cell lines. Values are presented as the mean ± standard error (n = 3). hEGF, human epidermal growth factor

Article Snippet: ELISA assay The protein samples were diluted at a ratio of 1:20 before each assay and were evaluated using a commercially available hEGF ELISA kit (R&D Systems Inc., USA) according to the manufacturer’s instructions.

Techniques: Incubation, Suspension, Transgenic Assay

Journal: Journal of Plant Biotechnology

Article Title: Production of human epidermal growth factor in cell suspension cultures of Centella asiatica (L.) Urban using balloon-type bubble bioreactors

doi: 10.5010/jpb.2024.51.020.206

Figure Lengend Snippet: Fig. 9 Growth index and hEGF expression of the transgenic cell line EK2 line in semi-solid and liquid cultures. Growth index was determined using the formula: fresh weight of the cells - dry weight of the cells/dry weight of cells. Values are presented as the mean ± standard error (n = 6). Statistical analysis of the data was performed using an independent sample t-test to compare the mean values between two groups. *** - significantly different at a p-value of 0.05. hEGF, human epidermal growth factor

Article Snippet: ELISA assay The protein samples were diluted at a ratio of 1:20 before each assay and were evaluated using a commercially available hEGF ELISA kit (R&D Systems Inc., USA) according to the manufacturer’s instructions.

Techniques: Expressing, Transgenic Assay

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Pomegranate: A review of the heavenly healer’s past, present, and future

doi: 10.22038/IJBMS.2023.72816.15844

Figure Lengend Snippet: Therapeutic potential of Punica granatum on the respiratory system

Article Snippet: Viral , Antiviral activity against SARS-Cov-2 , Punicalagin , 3CL protease assay kit (BPS Bioscience) , ↓Activity of the virus 3CL protease; IC50= 6.192 μg/ml , ( ) .

Techniques: Activity Assay, Software, Binding Assay, Modification, Virus, ABTS Assay, Screening Assay, Protease Assay, In Vitro, Colony Assay, Migration, Flow Cytometry, Membrane, Labeling, Activation Assay, Expressing, Incubation, Enzyme-linked Immunosorbent Assay, TUNEL Assay, Injection, Isolation, Plant Extract, Protein Concentration

Journal: Antioxidants

Article Title: Endothelial Notch Signaling Regulates the Function of the Retinal Pigment Epithelial Barrier via EC Angiocrine Signaling

doi: 10.3390/antiox12111979

Figure Lengend Snippet: Primer sequences used in this study.

Article Snippet: Then, the concentration of secreted HBEGF in the media was measured using the human HBEGF ELISA kit (Boster, Wuhan, China).

Techniques: Sequencing

Journal: Antioxidants

Article Title: Endothelial Notch Signaling Regulates the Function of the Retinal Pigment Epithelial Barrier via EC Angiocrine Signaling

doi: 10.3390/antiox12111979

Figure Lengend Snippet: Endothelial Notch signaling affects RPE barrier function by regulating the HBEGF paracrine of EC. ( A ) Analysis for previously published transcriptome sequencing data of normal HUVECs and DAPT treated HUVECs: the GSEA results for ECM ( left ) and heatmap of the expression of ECM-related genes ( right ). ( B ) mRNA and protein levels of HBEGF in normal HUVECs and DAPT-treated HUVECs. ( C ) mRNA and protein levels of HBEGF in normal HUVECs and NICD adenovirus-infected HUVECs. ( D ) The secretion levels of HBEGF protein in normal HUVECs and DAPT-treated HUVECs or NICD adenovirus-infected HUVECs were tested using an ELISA kit. ( E ) Evaluating the RPE barrier function in RPE cells cultured in normal medium, RPE cultured in the EC-conditioned medium, RPE cultured in Notch signaling-inhibited EC-conditioned medium, and RPE cultured in Notch signaling-inhibited EC-conditioned medium supplemented with recombinant HBEGF (r-HBEGF) using ZO-1 staining (top), fibronectin staining ( middle ), and collagen IV staining (bottom). The number of RPEs with intact tight junctions were quantitatively compared. Fibronectin- and collagen IV-positive areas were quantitatively compared. Scale bars: 50 µm. ( F ) Evaluating the RPE barrier function in the RPE, RPE with HUVECs, RPE with siHBEGF-treated HUVECs, and RPE with siNC-treated HUVECs using ZO-1 staining (top), fibronectin staining ( middle ), and collagen IV staining (bottom). The number of RPEs with intact tight junctions were quantitatively compared. Fibronectin- and collagen IV-positive areas were quantitatively compared. Scale bars: 50 µm. Three biological replicates were performed and values are presented as the mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Then, the concentration of secreted HBEGF in the media was measured using the human HBEGF ELISA kit (Boster, Wuhan, China).

Techniques: Sequencing, Expressing, Infection, Enzyme-linked Immunosorbent Assay, Cell Culture, Recombinant, Staining

Journal: Antioxidants

Article Title: Endothelial Notch Signaling Regulates the Function of the Retinal Pigment Epithelial Barrier via EC Angiocrine Signaling

doi: 10.3390/antiox12111979

Figure Lengend Snippet: r-HBEGF improved the RPE barrier dysfunction of Notch signaling deletion mice. ( A ) In the SI-induced RPE barrier disfunction model, whole-mount choroidal staining for EC-specific Notch signaling deletion mice CDH5-CRE-RBPJ f/f intravitreally injected with IgG or r-HBEGF, and control mice (RBPJ f/+ ) at p30, were analyzed using ZO-1 staining. The number of RPE cells with intact tight junctions were quantitatively compared. Red circles indicate the location of the optic disk. Scale bars: 50 µm. ( B ) In the laser-induced CNV model, whole-mount choroidal staining for EC-specific Notch signaling deletion mice CDH5-CRE-RBPJ f/f intravitreally injected with IgG or r-HBEGF and control mice (RBPJ f/+ ) were analyzed using IB-4 staining. IB4 positive areas were quantitatively compared. Three biological replicates were performed and values are presented as the mean ± SEM ( n = 3). Scale bars: 150 µm. * p < 0.05, ** p < 0.01.

Article Snippet: Then, the concentration of secreted HBEGF in the media was measured using the human HBEGF ELISA kit (Boster, Wuhan, China).

Techniques: Staining, Injection, Control

Journal: Antioxidants

Article Title: Endothelial Notch Signaling Regulates the Function of the Retinal Pigment Epithelial Barrier via EC Angiocrine Signaling

doi: 10.3390/antiox12111979

Figure Lengend Snippet: EC regulates MMP-9 expression in RPE cells via Endothelial Notch signaling-driven HBEGF secretion. ( A ) Protein interaction prediction analysis results of HBEGF and MMP-9, using the String database. ( B ) mRNA level, protein level, and enzyme activity level of MMP-9 in the RPE, RPE with HUVEC, and RPE with Notch signaling-inhibited HUVECs were tested via qRT-PCR, Western blot, and gelatin assay kit. ( C ) mRNA levels of MMP-9 in normal HUVECs and DAPT-treated HUVECs were tested via qRT-PCR. ( D , E ) mRNA and protein levels of MMP-9 in the RPE, RPE with HUVECs, RPE with siNC-treated HUVECs, and RPE with siHBEGF-treated HUVECs were tested via qRT-PCR and Western blot. ( F ) Protein levels of MMP-9 in RPE cells cultured in normal medium, RPE cultured in EC-conditioned medium, RPE cultured in Notch signaling-inhibited EC-conditioned medium, and RPE cultured in Notch signaling-inhibited EC-conditioned medium supplemented with recombinant HBEGF (r-HBEGF) were tested using Western blot. Three biological replicates were performed and values are presented as the mean ± SEM ( n = 3). NS, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Then, the concentration of secreted HBEGF in the media was measured using the human HBEGF ELISA kit (Boster, Wuhan, China).

Techniques: Expressing, Activity Assay, Quantitative RT-PCR, Western Blot, Cell Culture, Recombinant